Priority Research Paper Volume 15, Issue 20 pp 10821—10855

Proteomic quantification of native and ECM-enriched mouse ovaries reveals an age-dependent fibro-inflammatory signature

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Figure 2. Treatment with 0.1% SDS enriches for the ovarian ECM in 12.5 hours. Representative images of H&E (A), Hematoxylin (B), Hoechst (C), and PSR (D) stained ovarian tissue sections following treatment with 0.1% SDS for 0 h (native), 10 h, 12.5 h, or 15 h. Bottom row of each panel is optical zoom of boxed region from top row. Scale bar for top row for each panel = 400 μm. Scale bar for bottom row for each panel = 100 μm. N = 3–4 ovaries per group. Example of a corpus luteum indicated by ‡ in H&E-stained images. Example of region without nuclei indicated by arrows in Hematoxylin-stained images.