Research Paper Volume 15, Issue 20 pp 11412—11447
Molecular characterization of ferroptosis in soft tissue sarcoma constructs a prognostic and immunotherapeutic signature through experimental and bioinformatics analyses
Figure 10. Downregulation of LOX inhibits growth and increases ROS production in HT1080 and A204 cells. (A, B) qPCR analysis and Western blotting analysis were performed to detect the mRNA and protein levels of LOX in HT-1080 and A204 cells. (C, D) EdU assay was used to detect the proliferation of HT-1080 and A204 cells. (E, F) ROS levels were analyzed by flow cytometry in HT-1080 and A204 cells. (G, H) Transwell assay was performed to assess migration of HT-1080 and A204 cells. ***p < 0.001, ****p = 0.