Research Paper Volume 15, Issue 20 pp 11131—11151

Figure 1. Triptolide inhibits EOC cells proliferation, migration, and invasion. (A) SKOV3, A2780, and Ovcar8 were incubated with TPL for 24, 48, or 72 h at various doses (0 – 44.8 nM). The cell cytotoxicity assays were detected using CCK-8 analysis and IC50 was calculated by GraphPad Prism software 8.0. (B, C) SKOV3 and A2780 cells proliferation after treatment with various doses of TPL by EdU incorporation assay, (C) quantification of EdU-positive cells were analyzed. (D, E) Migration assay showed TPL dampened migration ability of EOC cells, Graph (E) represents the positive cell’s number of migrations. Scale bar: 100 μm. (F, G) Invasion assays showed TPL dampened invasion ability of EOC cells, Graph (G) represents the positive cell’s number of invasions. Scale bar: 100 μm. All results were derived from three independent repeated experiments and represented by mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control.