Research Paper Volume 16, Issue 4 pp 3185—3199
The high expression of TOP2A and MELK induces the occurrence of psoriasis
- 1 Department of Dermatology, China Aerospace Science and Industry Corporation 731 Hospital, Beijing 100074, China
Received: October 3, 2023 Accepted: December 7, 2023 Published: February 20, 2024
https://doi.org/10.18632/aging.205519How to Cite
Copyright: © 2024 Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Psoriasis is a chronic inflammatory skin disease. However, the influence of the TOP2A and MELK genes on psoriasis remains unclear.
Methods: Psoriasis datasets GSE166388 and GSE181318 were downloaded from the Gene Expression Omnibus (GEO) database generated from GPL570 and GPL22120. Differential gene expression (DEGs) was identified. Functional enrichment analysis, gene set enrichment analysis (GSEA), weighted gene co-expression network analysis (WGCNA), and immune infiltration analysis were conducted. The protein-protein interaction (PPI) network was constructed and analyzed. Gene expression heat map was generated. The most relevant diseases associated with core genes were determined through comparison with the Comparative Toxicogenomics Database (CTD) website. TargetScan was used to select miRNAs regulating central DEGs.
Results: A total of 773 DEGs were identified. According to Gene Ontology (GO) analysis, they were mainly enriched in mitochondrial gene expression, oxidative phosphorylation, mitochondrial envelope, mitochondria and ribosome. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that target cells were mainly enriched in metabolic pathways, proteasome, and oxidative phosphorylation. Seven core genes (TOP2A, NUF2, MELK, ASPM, DLGAP5, CCNA2, DEPDC1B) were obtained. The gene expression heatmap showed high expression of core genes (TOP2A, MELK) in psoriasis samples, while DEPDC1B, CCNA2, DLGAP5, NUF2, ASPM were lowly expressed in psoriasis samples. CTD analysis found that TOP2A and MELK were related to skin neoplasms, skin diseases, psoriasis, erythema, dermatitis, and infections.
Conclusion: TOP2A and MELK genes are highly expressed in psoriasis, and higher expression of TOP2A and MELK genes is associated with poorer prognosis.
Abbreviations
GEO: gene expression omnibus; DEGs: differential epigenetic genes; WGCNA: weighted gene co-expression network analysis; MAD: Median Absolute Deviation; TOM: topological overlap matrix; PPI: protein-protein interaction; STRING: Search Tool for the Retrieval of Interacting Genes; GO: gene ontology; KEGG: Kyoto Encyclopedia of Gene and Genome; GSEA: Gene set enrichment analysis; CTD: Comparative Toxicogenomics Database; FC: fold change; FDR: false discovery rate; MM: module membership.