Research Paper Volume 15, Issue 14 pp 7008—7022
KNTC1 and MCM2 are the molecular targets of gallbladder cancer
- 1 Department of Gastrointestinal Surgery, Beijing Rehabilitation Hospital, Capital Medical University, Badachu Xixia, Shijingshan 100144, Beijing, China
- 2 Department of Hepatobiliary Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China
Received: April 7, 2023 Accepted: June 15, 2023 Published: July 21, 2023
https://doi.org/10.18632/aging.204889How to Cite
Copyright: © 2023 Jia and Wang. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Gallbladder carcinoma is a malignant epithelial tumor of gallbladder with a high degree of malignancy. However, relationship between KNTC1 and MCM2 and gallbladder cancer is unclear.
Methods: GSE139682 and GSE202479 were downloaded from gene expression omnibus (GEO). Differentially expressed genes (DEGs) were screened. Functional enrichment analysis and gene set enrichment analysis (GSEA) were performed. Protein-protein interaction (PPI) Network was constructed and analyzed. Gene expression heat map was drawn. Comparative toxicogenomics database (CTD) analysis was performed to find diseases most related to core genes. TargetScan was performed for screening miRNAs that regulated central DEGs.
Results: 230 DEGs were identified. According to GObp analysis, they were mainly concentrated in regulation of ossification, regulation of spindle microtubule and centromere attachment, cytoskeleton tissue of cortical actin. According to GOcc analysis, they are mainly concentrated in plasma membrane part, cell junction, plasma membrane region and anterior membrane. According to GOmf analysis, they are mainly enriched in protein homodimerization activity, proximal promoter sequence-specific DNA binding and sulfur compound binding. KEGG showed that target genes were mainly enriched in Hippo signal pathway, p53 signal pathway and cancer pathway. KIFC2, TUBG1, RACGAP1, CHMP4C, SFN and MYH11 were identified as core genes. Gene expression heat map showed that KNTC1, MCM2, CKAP2, RACGAP1, CCNB1 were highly expressed in gallbladder carcinoma samples. CTD analysis showed that KNTC1, MCM2, CKAP2, RACGAP1, CCNB1 were associated with head and neck squamous cell carcinoma, necrosis, inflammation and hepatomegaly.
Conclusions: KNTC1 and MCM2 are highly expressed in gallbladder cancer. Higher expression level correlates with worse prognosis.
Abbreviations
GBC: gallbladder carcinoma; GEO: gene expression omnibus; DEGs: differential epigenetic genes; PPI: protein-protein interaction; GSEA: gene set enrichment analysis; CTD: comparative toxicogenomics database; FC: fold change; FDR: false discovery rate; STRING: Search Tool for the Retrieval of Interacting Genes; GO: gene ontology; KEGG: Kyoto Encyclopedia of Gene and Genome; RZZ: Rod-Zwilch-ZW10.