Research Paper Volume 16, Issue 8 pp 7217—7248

Figure 4. Hepatocyte-specific METTL3 homozygous ablation by Alb-iCre mice (GPT) causes apoptosis in mouse hepatocytes. (A, B) Representative TUNEL staining photographs (A) and quantification of TUNEL-positive hepatocytes of paraffin-embedded liver sections from control mice and METTL3^Δhep mice (GPT) at 7 or 14 days after birth. Scale bar = 100 μm. (C) qRT-PCR analysis of the expression of apoptosis-related genes in the liver of METTL3^Δhep mice (GPT). (D) Hierarchical clustering of differentially expressed genes (DEG) related to apoptosis in the liver of control mice and METTL3^Δhep mice (GPT) at 1 week after birth from RNA-seq data deposited in GEO under accession number GSE198512 [17]. In the cluster heatmap, class comparison and hierarchical clustering of DEGs involved in apoptosis in mouse livers were performed between control mice and METTL3^Δhep mice (GPT) at 7 days after birth. Genes with increased and reduced expressions are shown in red and blue, respectively. (E) GO analysis of up- and downregulated genes related to apoptosis (from RNA-seq data deposited in GEO under accession number GSE198512) in the liver of control mice and METTL3^Δhep mice (GPT) at 1 week after birth.