Research Paper Volume 16, Issue 3 pp 2362—2384

Figure 2. Volcano plot and heatmap of differentially expressed genes and the functional enrichment of KEGG and GO. (A) Volcano plot was constructed using the fold change values and P-adjust; red indicates upregulation, while blue indicates downregulation. (B) Heatmap of the differential gene expression; different colors represent the trend of gene expression in different tissues. The top 50 upregulated genes and the top 50 downregulated genes are shown in this figure. (C–F) The enriched KEGG signaling pathways and GO functions were selected to demonstrate the primary biological actions of major potential mRNAs. The abscissa indicates the gene ratio, and the enriched pathways are presented in the ordinate. Colors indicate the significance of differential enrichment, and the size of the circles indicates the number of genes. In the enrichment results, p < 0.05 and FDR <0.05 were considered meaningful pathways (enrichment score with −log10 (P) of more than 1.3).