Research Paper Volume 16, Issue 3 pp 2181—2193

Figure 7. EPI inhibits autophagy in H9C2 cells. (A) Cell viability of H9C2 cells. (B) Representative images of GFP-LC3 green fluorescence and lysosomal red fluorescence superimposed staining in H9C2 cells. (C) Relative quantitative positivity of GFP-LC3. (D) Western blot analysis of USP14, LC3 I, LC3 II and FTH1. (E) Co-IP analysis of ubiquitination of K63 on Beclin 1. Data (n=3) are expressed as mean ± SD, *P < 0.05, **P < 0.01.