Research Paper Volume 16, Issue 3 pp 2181—2193

(-)-Epicatechin protects against myocardial ischemia/reperfusion injury via autophagy-dependent ferroptosis

class="figure-viewer-img"

Figure 4. EPI increases the viability and antioxidant properties of H9C2 cells. (A) Cell viability of H9C2 cells. (B) The activity of SOD in H9C2 cells. (C) The content of MDA in H9c2 cells. (D) The concentration of Fe2+ in H9C2 cells. (E) The fluorescent intensity of ROS (probe-DCF) in H9C2 cells. (F) Histogram of ROS content of H9C2 cells. 2.5-EPI: 2.5 μM EPI; 5-EPI: 5 μM; 10-EPI: 10 μM EPI. Data (n=3) are expressed as mean ± SD, *P < 0.05 compared with OGD/R group, **P < 0.01 compared with OGD/R group.