Research Paper Volume 16, Issue 3 pp 2181—2193

Figure 3. EPI regulates the expression of autophagy and ferroptosis related proteins in cardiac tissues. (A) Western blot analysis of USP14, Beclin 1, NCOA4, LC3 I, LC3 II, FTH1 and TfR1, Co-IP analysis of ubiquitination of K63 on Beclin 1. (B) Densitometric analysis of the bands was presented as the relative ratio of USP14, Beclin 1, NCOA4, LC3 I, LC3 II, FTH1, TfR1. (C) Densitometric analysis of the bands was presented as the relative ratio of K63 ubiquitination on Beclin 1. I/R + L-EPI: 1 mg/kg/day of EPI; I/R + H-EPI: 2 mg/kg/day of EPI. Data (n=3) are expressed as mean ± SD, *P < 0.05 compared with I/R group, **P < 0.01 compared with I/R group.