Research Paper Volume 16, Issue 2 pp 1352—1373

Figure 4. miR-141-3p repressed H/R-induced autophagy and alleviated PMVEC injury. (A) The mRFP-GFP-LC3 dual fluorescence system was used to trace autophagy formation in PMVECs treated with H/R and miR-141-3p mimic. (B) The expression of LC3-I/LC3-II in H/R-exposed PMVECs treated with miR-141-3p mimic detected by Western blot. (C) The proliferation of H/R-exposed PMVECs treated with miR-141-3p mimic detected by CCK-8 assay under normoxia or H/R. (D) The migration of H/R-exposed PMVECs treated with miR-141-3p mimic detected by Transwell assay. (E) The apoptosis of H/R-exposed PMVECs treated with miR-141-3p mimic detected by flow cytometry. (F) The expression of Bax, Bcl-2, cleaved caspase-3 and caspase-3 in H/R-exposed PMVECs treated with miR-141-3p mimic detected by Western blot. (G) The expression of miR-141-3p and SIRT1 detected by RT-qPCR in H/R-exposed PMVECs treated with miR-141-3p mimic or combined with Rapamycin. (H) mRFP-GFP-LC3 dual fluorescence system was used to trace autophagy formation in H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with Rapamycin. (I) The proliferation of H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with Rapamycin detected by CCK-8 assay. (J) The migration of H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with Rapamycin detected by Transwell assay. (K) The apoptosis of H/R-exposed PMVECs treated with miR-141-3p mimic or combined with Rapamycin detected by flow cytometry. (L) The expression of Bax, Bcl-2, cleaved caspase-3 and caspase-3 in H/R-exposed PMVECs treated with miR-141-3p mimic alone or combined with Rapamycin detected by Western blot. Measurement data are expressed as mean ± standard deviation. Changes between multiple groups were compared using one-way ANOVA and Tukey’s multiple comparison test. Two-way ANOVA was used for data comparison at different time points. * p < 0.05. The cell experiments were repeated three times.