Research Paper Volume 16, Issue 2 pp 1352—1373

Figure 3. miR-141-3p targeted SIRT1 in H/R-treated PMVECs. (A) miR-141-3p had a binding site in the 3’UTR of SIRT1, predicted by the TargetScan website. (B) miR-141-3p expression detected by RT-qPCR under normoxia or H/R. (C) Binding of miR-141-3p and SIRT1 confirmed by dual luciferase assays. (D) Binding of miR-141-3p and SIRT1 detected by RIP. (E) Binding of miR-141-3p and SIRT1 detected by RNA pull-down. (F) Expression of miR-141-3p and SIRT1 detected by RT-qPCR in H/R-treated PMVECs with miR-141-3p mimic under normoxia or H/R. Measurement data are expressed as mean ± standard deviation. Differences between 2 groups of data were compared using an unpaired t-test. Data among multiple groups were compared using one-way ANOVA and Tukey’s multiple comparison test. * p < 0.05. The cell experiments were repeated three times.