Research Paper Volume 16, Issue 1 pp 928—947

Figure 9. LOXL1 expression in TNF-α-stimulated SW982 cells. (A) LOXL1, IL-6, INOS and COX2 mRNA expression levels were analyzed using qPCR. (B) SW982 cells were cultured and stimulated with TNF-α (20ng/mL). LOXL1, IL-6, INOS and COX2 protein levels were detected using western blot at the indicated time. (C) Quantitative analysis of western blot results. (D, E) qPCR and western blot were used to verify the efficiency of downregulation of LOXL1 gene expression by small interfering RNA. (F) Quantitative analysis of western blot results. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^***P < 0.0001.