Research Paper Volume 15, Issue 23 pp 14457—14472

Figure 6. miR-203a-3p exerted biological functions on chondrocytes by targeting MYD88. (A–C) Co-immunofluorescence staining of MMP13 (green) / Collagen II (red) and the relative quantification of positive cells in LPS-induced chondrocytes following transfection with miR-203a-3p inhibitor, si-MYD88, or co-transfection. (D–F) WB analysis and relative quantification showing the expression levels of cartilage matrix degradation related proteins (MMP3, MMP13, ADAMST-5, Collagen II, SOX9 and Aggrecan). (G, H) TUNEL staining showing the chondrocyte apoptosis and quantitative estimation of the number of TUNEL positive cells. (I, J) WB analysis and relative quantification showing the expression levels of apoptosis-related proteins (Bcl-2, Bax and Cleaved caspase-3) after transfection. (K–M) WB analysis and the relative quantification showing the expression levels of pyroptosis related proteins (NLRP3, ASC, Cleaved caspase-1, GSDMD, IL-1β, and IL-18). All experiments were performed in triplicated and data were presented as the mean±SD, n=3 per group. *P<0.05, **P<0.01.