Research Paper Volume 15, Issue 23 pp 14457—14472
MiR-203a-3p attenuates apoptosis and pyroptosis of chondrocytes by regulating the MYD88/NF-κB pathway to alleviate osteoarthritis progression
Figure 2. miR-203a-3p directly targeted MYD88. (A, B) Three online databases were used to predict the miR-203a-3p target genes. (C) Schematic representation of the MYD88 3’-UTR. A putative miR-203a-3p-binding site existed in the MYD88 mRNA. (D) The relative luciferase activity between cells was compared following co-transfection of MYD88-WT/ MYD88-MUT vectors with miR-203a-3p mimics and consistent negative controls. (E, F) qRT-PCR showing the mRNA expression of miR-203a-3p and MYD88 following transfection with miR-203a-3p mimic, inhibitor and consistent negative controls. (G–J) WB and immunofluorescence analysis showing the protein expression of MYD88 following transfection. All experiments were performed in triplicated and data were presented as the mean±SD, n=3 per group. *P<0.05, **P<0.01.