Research Paper Volume 16, Issue 1 pp 106—128

Figure 7. The effects of JPYSF on the expression of NAD⁺ biosynthesis-related enzymes in HK-2 cells. (A) Representative Western blot images of QPRT, NAPRT1, NMNAT1, NRK1, and NAMPT expression in HK-2 cells stimulated with TGF-β1 at the concentrations of 0, 2, 5, and 10 ng/mL. (B–F) Quantitative analysis of QPRT, NAPRT1, NMNAT1, NRK1 and NAMPT expression in HK-2 cells stimulated with TGF-β1 at the concentrations of 0, 2, 5 and 10 ng/mL, normalized to β-actin content (n = 3). (G) Representative Western blot images of QPRT, NMNAT1, and NRK1 expression in HK-2 cells with TGF-β1 or/and JPYSF stimulation. (H–J) Quantitative analysis of QPRT, NMNAT1, and NRK1 expression in HK-2 cells with TGF-β1 or/and JPYSF stimulation, normalized to β-actin content (n = 3). Data are expressed as mean ± SEM (^nsp > 0.05, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 between the indicated two groups).