Research Paper Volume 15, Issue 23 pp 14306—14322
DOK7, a target of miR-299-5p, suppresses the progression of bladder cancer
Figure 6. DOK7 negatively regulates JAK/STAT3 pathway in BLCA cells. (A) GESA analysis of TCGA BLCA dataset revealed that the low expression of DOK7 is associated with JAK/STAT signaling pathway activation; (B) WB detection of DOK7 protein level and phosphorylation levels of JAK1-3 as indicated in different groups (sh-NC+NC inhibitor, sh-DOK7+NC inhibitor and sh-DOK7+miR-299-5p inhibitor) of T24 and RT4 cells. (C) WB detection of DOK7 protein level and phosphorylation levels of JAK1-3 as indicated in different groups (vector+miR-NC, DOK7-vector+miR-NC and DOK7-vector+miR-299-5p mimic) of T24 and RT4 cells.