Research Paper Volume 15, Issue 23 pp 14172—14191

Gypenoside IX restores Akt/GSK-3β pathway and alleviates Alzheimer’s disease-like neuropathology and cognitive deficits

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Figure 4. Effects of GP on neuronal dendrites and synaptic plasticity-related proteins in the hippocampus neurons. (A) Representative images of immunofluorescence staining for MAP-2 labeled neuronal dendrites; scale bar = 20 μm. (B) Sholl analysis of dendritic arborization of neurons in (A). (C) Representative Western blots of PSD-95, synaptophysin, and GluR2. (DF) Quantitative analysis of PSD-95, GluR2, and synaptophysin after normalization to the β-actin signal. Data represent mean ± SEM, p-value significance is calculated from one-way ANOVA, n = 3. *P < 0.05, ***P < 0.001 vs. control group, NS, no significance. #P < 0.05, ##P < 0.01 vs. GP10, $P < 0.05, $$P < 0.01 vs. GP5.