Research Paper Volume 15, Issue 23 pp 13961—13979

Figure 5. CTSV cleaves off fibronectin, E-cadherin, and N-cadherin. (A, B) Stable CTSV-HA A549 cells were subjected to coimmunoprecipitation assays using anti-fibronectin, anti-E-cadherin, and anti-N-cadherin antibodies, followed by Western blotting thrice. The grouping of blots cropped from different parts of the different gels. (C) Lysates of A549 cells were subjected to IP using anti-fibronectin, anti-E-cadherin, and anti-N-cadherin antibodies and control IgG, then detected by immunoblotting. (D–F) 1 μg recombinant human fibronectin, E-cadherin, and N-cadherin were incubated with 1 μg recombinant human CTSV at 37°C for the indicated periods. Fibronectin, E-cadherin, and N-cadherin fragments were detected using anti-fibronectin, anti-E-cadherin, and anti-N-cadherin antibodies. HA is shown as a control. The grouping of blots cropped from different parts of the same gels.