Research Paper Volume 15, Issue 23 pp 13961—13979

Figure 3. CTSV promotes lung cancer metastasis by cleaving off adhesion proteins. (A–C) Immunofluorescence analysis was performed using an anti-actin antibody in the indicated stable cells. Scale bar, 50 μm. (D) The indicated A549/NCI-H1975 cells were analyzed by adhesion assay. (E) Protein mass spectrometry analysis of CTSV binding partner candidates in the indicated stable cells. (F, G) A549 and NCI-H1975 cells expressing CTSV-HA-medium and CTSV-HA-high were analyzed by Western blotting using anti-fibronectin, anti-E-cadherin and anti-N-cadherin antibodies. (H–J) A549, NCI-H1975, and NCI-H292 cells transfected with negative control plasmids, shCTSV#1 or shCTSV#3 for 48 h were analyzed by Western blotting using anti-fibronectin, anti-E-cadherin and anti-N-cadherin antibodies. The grouping of blots cropped from different parts of the different gels. Data are shown as the mean ± SD and repeated for three biologically independent experiments. P-values are shown; two-tailed Student’s t-test. Abbreviation: NC: negative control shRNA. Vector, vector-only control. The representative data are repeated for three biologically independent experiments. Student’s t-tests were used for data analysis; ^*P < 0.05, ^**P-value < 0.01, ^***P-value < 0.001 and ^****P-value < 0.0001.