Research Paper Volume 15, Issue 22 pp 13452—13470

Figure 2. Fer-1 suppressed H₂O₂-induced catabolism and ferroptosis of chondrocytes. (A) Quantitative qRT-PCR of the gene expression of Col2α1, ACAN, MMP-13 and Adamts-5 under Fer-1 (2 μM) and H₂O₂ (500 μM) cultured conditions treatment. (B) Western blot analyses of Col2α1, ACAN, MMP-13 and Adamts-5 under Fer-1 and H₂O₂ cultured conditions treatment. (C) Quantitative western blot of Col2α1, ACAN, MMP-13 and Adamts-5 under Fer-1 and H₂O₂ cultured conditions treatment. n = 3. (D) Representative ROS, Lipid-ROS and Fe²⁺ fluorescence detection under Fer-1 and H₂O₂ cultured conditions treatment. (E) Quantitative ROS, Lipid-ROS and Fe²⁺ fluorescence under Fer-1 and H₂O₂ cultured conditions treatment. n = 3. ROS and Fe²⁺: Scale bars, 50 μm. Lipid-ROS: Scale bars, 100 μm. (F) Quantitative analysis of GSH and MDA under Fer-1 and H₂O₂ cultured conditions treatment. (G) Quantitative qRT-PCR of the gene expression of GPX-4, SLC7A11, ACSL4 and COX-2 under Fer-1 and H₂O₂ cultured conditions treatment. (H) Western blot analyses of GPX-4, SLC7A11, ACSL4 and COX-2 under Fer-1 and H₂O₂ cultured conditions treatment. (I) Quantitative western blot of GPX-4, SLC7A11, ACSL4 and COX-2 under Fer-1 and H₂O₂ cultured conditions treatment. n = 3. (All quantified data are shown as mean ± SEM; #p < 0.05, ##p < 0.01, *p < 0.05, **p < 0.01, by one-way ANOVA followed by the Tukey-Kramer test, #: CON vs H₂O₂, *: H₂O₂ vs Fer-1+ H₂O₂).