Research Paper Volume 15, Issue 22 pp 13194—13212
Electroacupuncture ameliorates AOM/DSS-induced mice colorectal cancer by inhibiting inflammation and promoting autophagy via the SIRT1/miR-215/Atg14 axis
Figure 4. SIRT1 represses miR-215 expression. (A) Venn diagram showing miRNAs co-regulated by Model vs. EA and Model vs. resveratrol; (B) Volcano plots showing differentially expressed miRNAs (Red: high expression; green: low expression). (C) Differential gene expression. (D) Relative expression level of miR-215 in the mice colorectal tissue by RT-qPCR detection. (E) GO enrichment assessment. (F) KEGG enrichment assessment. (G) mRNA expression of SIRT1 after overexpression of SIRT1 in SW480 and HCT116. (H) mRNA expression of SIRT1 after inhibition of SIRT1 in SW480 and HCT116. (I) mRNA expression of miR-215 after overexpression of SIRT1 in SW480 and HCT116. (J) mRNA expression of miR-215 after inhibition of SIRT1 in SW480 and HCT116. Three mice were analyzed in each group. Data indicate mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001, compared to the control group.