Research Paper Volume 15, Issue 22 pp 13194—13212
Electroacupuncture ameliorates AOM/DSS-induced mice colorectal cancer by inhibiting inflammation and promoting autophagy via the SIRT1/miR-215/Atg14 axis
Figure 3. EA promotes SIRT1 expression and autophagy. (A) Western blotting and RT-qPCR and evaluation of SIRT1 expression in colon tissues. (B) Representative Western blotting graphs showing the expression of P62, Beclin1, and LC3 in colon tissues. (C) Quantitation of the expression contents of P62, Beclin1, and LC3. (D) RT-qPCR evaluation of the expression of P62, Beclin1 and LC3. Data are presented as mean ± SD (n = 8). #p < 0.05, ##p < 0.01, ###p < 0.001, compared to the control group; *p < 0.05, **p < 0.01, and ***p < 0.001, compared to the model group.