Research Paper Volume 15, Issue 22 pp 13163—13175

Hypoxia-regulated exosomes mediate M2 macrophage polarization and promote metastasis in chondrosarcoma

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Figure 1. Isolation of exosomes derived from chondrosarcoma cells. (A) Identical concentrations of SW1353 cells were seeded under normoxic (O2 = 20%) and hypoxic (O2 = 1%) conditions for 48 h. Exosome (SW1353-N-exo and SW1353-H-exo) size was the characterization of exosomes by Nanoparticle Tracking Analysis (NTA). (B, C) Western blot analysis for exosomal proteins CD9, ALIX, TSG101, CD63, HSP70. All replicate number ≧ 3. *p < 0.05 compared with the SW1353-N-exo group.