Research Paper Volume 15, Issue 15 pp 7794—7810

Figure 3. Inhibition of Hmgcs2 aggravates inflammatory response and reduces M2 macrophages in the heart of CLP mice. (A) The concentrations of the inflammatory factor IL-1β in the serum of mice in each group (ng/ml). (B) The concentration of IL-6 in the serum of mice in each group (ng/ml). (C) The concentration of IL-10 in the serum of mice in each group (ng/ml). (D) The concentrations of TNF-α in the serum of mice in each group (pg/ml). (E) GO enrichment results are exhibited in a bubble plot, with bubble size representing how many enriched genes are and different colors representing different kinds of GO units. (F) The bar graph exhibits the enrichment results for the up-regulated genes GO enrichment analysis results. (G) GO enrichment analysis results of down-regulated genes. (H) Immuno-fluorescence co-localization of heart samples from CLP mice. Where green fluorescence represents CD163, red fluorescence represents Arg1, blue represents nuclei (DAPI), and the bottom is a composite (merge) diagram (magnification 400×). Sham: sham group (n = 6); Sham + ASO: sham surgery plus ASO administration group (n = 6); CLP: septic myocarditis model group in CLP operated mice (n = 6); CLP + ASO: CLP plus ASO administration group (n = 6). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001: vs. sham group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001: vs. CLP group.