Research Paper Volume 15, Issue 14 pp 6933—6949
Combined surface functionalization of MSC membrane and PDA inhibits neurotoxicity induced by Fe3O4 in mice based on apoptosis and autophagy through the ASK1/JNK signaling pathway
Figure 5. Effects of MSCM-Fe3O4@PDA, Fe3O4@PDA and Fe3O4 NPs on autophagy. (A) Ultrastructure of autophagy in neurons under electron microscope (autophagy shown by arrow). (B) Detection of p62, LC3, and Beclin 1 mRNA levels by RT-qPCR. (C) The representative pictures and statistical chart of LC3, p62 and Beclin 1 protein by immunohistochemistry. (D) Detection of LC3-I, LC3-II, p62 and Beclin 1 protein by western blot. ap < 0.05 when compared with control, bp < 0.05 when compared with MSCM-Fe3O4@PDA, cp < 0.05 when compared with Fe3O4@PDA.