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Research Paper Volume 15, Issue 14 pp 6933—6949

Combined surface functionalization of MSC membrane and PDA inhibits neurotoxicity induced by Fe3O4 in mice based on apoptosis and autophagy through the ASK1/JNK signaling pathway

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Figure 4. Effects of MSCM-Fe3O4@PDA, Fe3O4@PDA and Fe3O4 NPs on apoptosis. (A) TUNEL staining to observe the apoptosis of neuron cells in mice brain. (B) Detection of Caspase-3, Caspase-8, Caspase-9, Bax and Bcl-2 mRNA levels by RT-qPCR. (C) The representative pictures and statistical chart of Cleaved Caspase-3, Cleaved Caspase-8, Cleaved Caspase-9, Bax and Bcl-2 protein by immunohistochemistry. (D) Detection of Cleaved Caspase-3, Cleaved Caspase-8, Cleaved Caspase-9, Bax and Bcl-2 protein by western blot. ap < 0.05 when compared with control, bp < 0.05 when compared with MSCM-Fe3O4@PDA, cp < 0.05 when compared with Fe3O4@PDA.