Research Paper Volume 15, Issue 15 pp 7408—7423
Senescence-related gene c-Myc affects bladder cancer cell senescence by interacting with HSP90B1 to regulate cisplatin sensitivity
Figure 7. c-Myc/HSP90B1 interaction regulates cisplatin sensitivity by affecting bladder cancer cell senescence. (A) Knockdown of HSP90B1 to analyze its effect on c-Myc regulation of T24 cells proliferation ability. (B) Knockdown ofHSP90B1 to analyze its effect on c-Myc regulation of T24 cells senescence. (C) Knockdown of HSP90B1 to analyze its effect on c-Myc regulation of 5637 cells proliferation ability. (D) Knockdown of HSP90B1 to analyze its effect on c-Myc regulation of 5637 cells senescence. (E, F) Knockdown of HSP90B1 to analyze its effect on c-Myc-regulated cisplatin sensitivity in T24 and 5637 cells. (G) CO--IP analysis with HSP90B1 or c-Myc antibody in T24 cells. (H) Western blot analysis of the effect of HSP90B1 on c-Myc-regulated p21 signaling pathway.