Research Paper Volume 15, Issue 15 pp 7408—7423
Senescence-related gene c-Myc affects bladder cancer cell senescence by interacting with HSP90B1 to regulate cisplatin sensitivity
Figure 4. Knockdown of c-Myc promotes bladder cancer cell senescence and affects the sensitivity of cisplatin chemotherapy. (A) The expression of c-Myc protein was reduced in T24 and 5637 cells. (B, C) CCK8 and clonogenic assay was used to detect the effect of c-Myc knockdown on the proliferation of bladder cancer T24 cells. (D) The expression of c-Myc protein was decreased in T24 cells, and its effect on the expression of PCNA and P21 protein was detected. (E, F) CCK8 and clonogenic assay was used to detect the effect of c-Myc knockdown on the proliferation of bladder cancer 5637 cells. (G) c-Myc was knocked down in T24 cells, and its effect on cell senescence was analyzed by SA-β-gal. (H) c-Myc was knocked down in T24 cells to detect its effect on the sensitivity of cisplatin chemotherapy. (I) c-Myc was knocked down in 5637 cells, and its effect on cell senescence was analyzed by SA-β-gal. (J) c-Myc was knocked down in 5637 cells to detect its effect on the sensitivity of cisplatin chemotherapy.