Research Paper Volume 15, Issue 12 pp 5798—5825

Figure 15. Knockdown of trim5 or trim34 attenuated the proliferation, invasion, and migration capabilities of glioma cells. (A–D) Cell proliferation was evaluated in U87 and U251 cells with or without Trim5 or Trim34 knockdown by CCK-8 assay, Trim5 or Trim34 shRNA #1 versus control shRNA, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001; Trim5 or Trim34 shRNA #2 versus control shRNA; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001. (E, F) Colony formation capability was examined in U87 and U251 cells with or without Trim5 or Trim34 knockdown by colony-formation assay, Trim5 or Trim34 shRNA versus control shRNA, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 (G–J) Cell migration and invasion ability was evaluated in U87 and U251 cells with or without Trim5 or Trim34 knockdown by transwell assay, Trim5 or Trim34 shRNA versus control shRNA, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.