Research Paper Volume 15, Issue 13 pp 6163—6178

Figure 3. Inhibiting PRMT5 impairs VEGFR2/Akt/eNOS signaling axis. (A) The HUVECs were incubated with vehicle or GSK591 (10μM) for five days, and the cells were serum starved and treated with VEGF (50ng/mL) at different time points. The cells were harvested, and the indicated protein expression levels were assessed by Western blotting (n=3). GAPDH served as an internal control. (B) The HUVECs were infected with lentivirus containing scramble-shRNA or PRMT5-shRNA2, and the cells were serum starved. The cells were stimulated with VEGF (50 ng/mL), and the indicated protein expression levels were assessed by Western blotting (n=3). The PRMT5 activity was determined by the expression of SDMA.