Research Paper Volume 16, Issue 4 pp 3973—3988
MicroRNA-320a enhances LRWD1 expression through the AGO2/FXR1-dependent pathway to affect cell behaviors and the oxidative stress response in human testicular embryonic carcinoma cells
Figure 3. Knockdown of AGO2 and FXR1 was correlated with a reduction in miR-320a-mediated upregulation of LRWD1. The effect of the presence and absence of AGO2 and FXR1 expression in regulating (A) LRWD1 expression by luciferase assay. (B) LRWD1 gene expression by qRT-PCR using AGO2- and FXR1-specific shRNAs (shLuc: negative control; shAGO2: AGO2 shRNA; shFXR1: FXR1 shRNA; *p < 0.05, **p < 0.01, ***p < 0.001 compared with the vector-only control group; Abbreviation: NS, no significance).