Research Paper Volume 16, Issue 1 pp 844—856
Melatonin regulates autophagy in granulosa cells from patients with premature ovarian insufficiency via activating Foxo3a
Figure 4. Improvement in cell proliferation and survival via activated autophagy with MT treatment. (A) Representative EdU staining results and analysis for cell proliferation in POI-GCs after MT treatment (n = 5). Scale bar, 100 μm. (B) Flow cytometry analysis of apoptotic rates in POI-GCs after MT treatment (n = 3). (C) Analysis of ROS production in POI-GCs after MT (n = 3). (D) Representative IF staining and analysis of autophagy in POI-GCs after 3-MA treatment with or without MT (n = 5). (E) EdU results of POI-GCs. (F) Analysis of ROS production in POI-GCs after 3-MA treatment with or without MT. **p < 0.01, ***p < 0.001.