Research Paper Volume 16, Issue 1 pp 648—664

Danggui-Shaoyao-San (DSS) ameliorates the progression of osteoarthritis via suppressing the NF-κB signaling pathway: an in vitro and in vivo study combined with bioinformatics analysis

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Figure 7. DSS inhibited LPS mediated activation of the NF-κB pathway. The chondrocytes were pretreated with DSS for 24 h and stimulated with or without LPS for 2 h. (A) The protein levels of p-IκBα, IκBα, p-p65 and p65 were assessed by western blot. (B) The nuclei translocation of p65 was analyzed by the immunofluorescence staining. #p < 0.05, ###p < 0.001 vs. control group; *p < 0.05, **p < 0.01 vs. LPS alone treatment group, n = 3.