Research Paper Volume 15, Issue 24 pp 15161—15182
Reveal the correlation between hub hypoxia/immune-related genes and immunity and diagnosis, and the effect of SAP30 on cell apoptosis, ROS and MDA production in cerebral ischemic stroke
Figure 9. The relative content of SAP30, ROS and MDA and the apoptosis rate in HMC3-OGD/R model group and HMC3 control group. (A) The relative expression level of SAP30 in HMC3-OGD/R model group and HMC3 control group was detected by real time-PCR; (B) Fluorescence value of ROS in HMC3-OGD/R model group and HMC3 control group; (C) Content of MDA in HMC3-OGD/R model group and HMC3 control group; (D) Apoptosis rate in HMC3 control group was detected by flow cytometry; (E) Apoptosis rate in HMC3-OGD/R model group was detected by flow cytometry; (F) Histogram of apoptosis rate in HMC3-OGD/R model group and HMC3 control group. ****P < 0.0001; Abbreviations: HMC3-NC: HMC3 control group; HMC3-OGD/R HMC3-OGD/R model group. Q1-2 and Q1-4 quadrants represent late apoptotic cells and early apoptotic cells, respectively.