Research Paper Volume 15, Issue 21 pp 12251—12263

Figure 3. PPI increases the sensitivity of liver cancer-resistant cells to sorafenib and inhibits their invasion and metastasis. (A, B) To measure chemotherapy efflux using flow cytometry, cells are first loaded with a fluorescently labeled chemotherapy drug sorafenib. The HCC cells were then analyzed by flow cytometry to measure the intracellular fluorescence intensity of the PPI or Sora treatment. Intracellular fluorescence intensity was measured using flow cytometry, and the efflux of chemotherapy drugs in cells was quantified and presented in the bar chart. Statistical significance is indicated by asterisks (^*P < 0.05, ^**P < 0.01, ^***P < 0.001). (C, D) The effects of PPI or Sora on the migration and invasion ability of liver cancer cells. (E, F) The bar graphs in panels C and D represent the mean ± standard error. ^*p < 0.05, ^**p < 0.01.