Research Paper Volume 15, Issue 21 pp 12209—12224
Effects of amyloid β (Aβ)42 and Gasdermin D on the progression of Alzheimer’s disease in vitro and in vivo through the regulation of astrocyte pyroptosis
Figure 2. Aβ42 induces astrocyte pyroptosis. (A) Astrocytes were stimulated with the 1 μmol Aβ42 for 48 h were harvested for PI staining and flow cytometry analysis. (B) Scanning electron microscopy was performed to observe astrocyte pyroptosis in the control and Aβ42 groups. (C) Real-time quantitative PCR was used to detect the expression of astrocyte pyroptosis-associated inflammasomes (NLRP1, NLRP3, NLRP4) in the control and Aβ42 groups. *p < 0.05 vs. control group. Abbreviations: Mv: microvilli; PS: pseudopods; CP: cell projection; Ch: cell hole; CO: cellular content.