Research Paper Volume 15, Issue 20 pp 11114—11130

Figure 2. NKD1 suppresses hypoxia-induced proliferation and migration of PASMCs via inhibiting oxidative stress. (A) PASMCs were transfected with Ad-Con or Ad-Nkd1 and then cultured in the condition of normoxia or hypoxia. Above treated PASMCs were stained with DHE. Representative pictures and the corresponding quantification of DHE fluorescence intensity were shown (post hoc for LSD test; n = 6 samples). Bar = 50 μm. Hypoxia-challenged PASMCs were transfected with Ad-Con or Ad-Nkd1 and then cultured with or without paraquat treatment. (B) Above treated PASMCs were stained with Ki-67 (green) and DAPI (blue). Representative pictures and the corresponding percentage of Ki-67-positive PASMCs were shown (post hoc for LSD test; n = 6 samples). Bar = 50 μm. (C) Migration of above-treated PASMCs was assessed by wound-healing assay (post hoc for LSD test; n = 6 samples). Bar = 200 μm. Data were shown as mean ± S.D. ^***P < 0.001 denoted statistical comparison between the two marked groups.