Research Paper Volume 15, Issue 20 pp 11052—11066
The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma
Figure 5. Overexpression of MAD1L1 partially rescued the diminished proliferative and invasive ability caused by silencing CHPF expression in glioma. (A, B) Flow cytometry assay showed the effects of MAD1L1 overexpression on U251 and U87 cells S conversion. (C) Western blot analysis showed the effects of MAD1L1 overexpression on the change of cycle-related proteins expression in U251 and U87 glioma cells. (D–F) CCK-8 and transwell assay showed the effects of MAD1L1 overexpression on U251 and U87 cells growth and migration. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.