Research Paper Volume 15, Issue 17 pp 8812—8832
SUMO1-regulated DBC1 promotes p53-dependent stress-induced apoptosis of lens epithelial cells
Figure 9. p53 overexpression overrides the effect of DBC1 absence in promoting oxidative stress-induced apoptosis. (A) DBC1 KO cells were transiently transfected with Flag-vector or Flag-p53 as indicated, then incubating with or without 40 mU GO for 5 hours followed by Calcein/PI Cell Viability/Cytotoxicity assay analysis on cell apoptosis. Scale bar, 100 μm. (B) Quantification of the PI-DNA puncta in panel A. *p < 0.05, ****p < 0.0001.