Research Paper Volume 15, Issue 14 pp 7308—7323

Subcellular localization of nucleolar protein 14 and its proliferative function mediated by miR-17-5p and E2F4 in pancreatic cancer

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Figure 3. miR17-5p mediates the promotive effect of NOP14 on the proliferation of PANC1 pancreatic cancer cell. (A) Expression level of miR17-5p after up- or downregulation of NOP14, as determined by qRT–PCR. (B) Expression level of miR17-5p after transfection with the miR17-5p analog and control (scramble), inhibitor (anti-17-5p) and control (anti-con), as determined by qRT–PCR. (C) Cell proliferation ability after upregulation of miR17-5p expression by the miR17-5p analog. (D) Cell proliferation ability after downregulation of miR17-5p expression by anti-miR17-5p. (E) Cell proliferation ability after downregulation of NOP14 and upregulation of miR17-5p. (F) Cell proliferation ability after upregulation of NOP14 and downregulation of miR17-5p. *P < 0.05, **P < 0.01, ***P < 0.001.