Research Paper Volume 15, Issue 14 pp 6658—6689

Figure 5. Transcriptomic analysis of NLRP3 inhibition. (A) Principal component analysis of the individual samples (young, middle-aged and middle-aged treated with MCC950 (NLRP3i)). (B) Volcano Plot comparing the saline- and MCC950-treated middle-aged podocyte transcriptomes; transcripts changed >2 and with a p-value > 0.05 are indicated in blue when down regulated upon NLRP3i and in red, when increased. (C) GSEA plot of the NLRP3 inflammasome gene set decreased upon treatment with MCC950. (D, E) Venn diagrams comparing the transcripts up-regulated in middle-aged podocyte and down-regulated upon NLRPi (D) and those down-regulated in the middle-aged and up-regulated upon NLRP3i (E) using the differentially expressed genes determined by the DSEQ2 analysis. (F–I) GSEA plots for the Reactome gene set cellular senescence comparing young to middle-aged (F) and middle-aged to middle-aged to NLRP3i-treated (G). Most differentially up- and down-regulated transcripts are compared along all three conditions in the histograms in panel H and I, respectively. (J–M) GSEA plots for the Rodwell aging kidney up (J) and down (I) gene sets comparing middle-aged to middle-aged to NLRP3i-treated. Most differentially expressed transcripts for either gene set are compared along all three conditions in the histograms (L, M).