Impaired energy metabolism of senescent muscle satellite cells is associated with oxidative modifications of glycolytic enzymes

Martín A. Baraibar; Janek Hyzewicz; Adelina Rogowska-Wrzesinska; Anne-Laure Bulteau; Carina Prip-Buus; Gillian Butler-Browne; Bertrand Friguet

doi:doi:10.18632/aging.101126

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Research Paper Volume 8, Issue 12 pp 3375—3389

Impaired energy metabolism of senescent muscle satellite cells is associated with oxidative modifications of glycolytic enzymes

Figure 1. Replicative senescence of human satellite cells in vitro. (A) Immunocytochemistry against desmin evidences morphological changes in senescent human myoblasts (SEN) when compared to young cells (CPD 30). Note the increased diameter and irregular shapes of the formers, as previously described [13]. (B) p16 protein levels during replicative senescence in human myoblasts. (C) Densitometric analysis of the p16/emerin ratio showed a significant increase (n=3; *P<0.001) in p16 levels during replicative senescence. P16 protein levels are expressed as relative values and shown as mean ± S.D.