Research Paper Volume 5, Issue 7 pp 539—550

Figure 2. Rapamycin has no major effects on mitochondrial protein levels. Representative subunits of each electron transport complex were detected by Western blotting using a cocktail of monoclonal antibodies from MitoSciences. Because the complex IV subunit was not detected using the cocktail, a separate COXIV antibody was also used. The identities of the probed subunits are as follows: complex I - NADH dehydrogenase 1 beta subcomplex 8 (NDUFB8); complex II - succinate dehydrogenase subunit B (SDHB); complex III - ubiquinol-cytochrome c reductase core protein 2 (UQCRC2); complex V -ATP synthase subunit alpha (ATP5A). Proteins were measured in (A) soleus or (B) gastrocnemius (gastroc) muscle following 2 weeks of rapamycin treatment. Data were obtained from C57BL/6 mice following an overnight fast.